Comparative study on the growth and yield of pleurotus ostreatus (jacq. fr.) mushroom on different substrates

Devendra Nirmalkar; Vijay Laxmi Naidu; Ranjana Shrivastava; Daneshwar Prasad

doi:10.59367/8393sz24

Authors

Devendra Nirmalkar Department of Botany, Govt. V. Y. T. PG Autonomous College Durg (Chhattisgarh).
Vijay Laxmi Naidu Department of Botany, Govt. V. Y. T. PG Autonomous College Durg (Chhattisgarh).
Ranjana Shrivastava Department of Botany, Govt. V. Y. T. PG Autonomous College Durg (Chhattisgarh).
Daneshwar Prasad Department of Botany, Govt. V. Y. T. PG Autonomous College Durg (Chhattisgarh).

DOI:

https://doi.org/10.59367/8393sz24

Keywords:

Cultivation, Biological efficiency, Moisture, FTIR

Abstract

Pleurotus ostreatus also known as oyster mushroom, oyster mushroom is a widely cultivated edible fungus that has various nutritional and medicinal benefits. In this paper, we report the cultivation of Pleurotus ostreatus on different substrates, such as paddy straw, soyabean straw, and redgram straw, and compare their growth parameters, such as yield, biological efficiency, and moisture content. We also perform Fourier transform infrared (FTIR) spectroscopy on the harvested mushrooms to analyze their chemical composition and functional groups. The results show that soyabean straw is the most suitable substrate for Pleurotus ostreatus cultivation, as it produces the highest yield (206.96 g/2030.85g), biological efficiency (62.20%), and moisture content (81.3%). paddy straw and redgram staw produce lower yields (174.02g/1771.36g and 120.03g/2458.76g, respectively), biological efficiencies (42.21% and 32.40%, respectively), and moisture contents (85.7% and 89.8%, respectively). The FTIR spectra of Pleurotus ostreatus reveal the presence of various functional groups, such as hydroxyl, amine, amide, and carbonyl, which indicate the presence of carbohydrates, proteins, and other organic compounds. The FTIR analysis also shows that the chemical composition of Pleurotus ostreatus varies depending on the substrate used for cultivation, which may affect its nutritional and medicinal properties.

References

Adapa, P. K., Schonenau, L. G., Canam, T., & Dumonceaux, T. (2011). Quantitative analysis of lignocellulosic components of non-treated and steam exploded barley, canola, oat and wheat straw using Fourier transform infrared spectroscopy. Journal of Agricultural Science and Technology, 177.

Adewoyin, A. G. and Anyandele, A. A. (2018). Comparative study of yield performance and nutrient composition of the edible mushroom Pleurotus pulmonarius cultivated on different substrates. African journal of plant science, 148- 154.

Adjapong A. O., Ansah K. D., Angfaarabung F. and Sintim H. O. (2015). Maize residue as a viable substrate for farm scale cultivation of oyster mushroom (Pleurotus ostreatus). Advances in Agriculture.

Agba M. O., Markson A. A., Oni J. O. & Bassey G. A. (2021). Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq. P. Kumm) cultivated on different agricultural wastes. Journal Bioscience Agricultural Research, 27(1): 2225-2233.

Ahmed, S. A., Kadam, J. A., Mane, V. P., Patil, S. S., & Baig, M. M. V. (2009). Biological efficiency and nutritional contents of Pleurotus florida (Mont.) Singer cultivated on different agro-wastes. Nature and science, 7(1), 44-48.

Akcay, C., & Yalcin, M. (2021). Morphological and chemical analysis of Hylotrupes bajulus (old house borer) larvae-damaged wood and its FTIR characterization. Cellulose, 28(3), 1295-1310.

Ali, N., Tabi, A. N. M., Zakil, F. A., Fauzai, W. N. F. M., & Hassan, O. (2013). Yield performance and biological efficiency of empty fruit bunch (EFB) and palm pressed fibre (PPF) as substrates for the cultivation of Pleurotus ostreatus. J. Teknol, 64, 93- 99.

Bari, E., Nazarnezhad, N., Kazemi, S. M., Ghanbary, M. A. T., Mohebby, B., Schmidt, O., & Clausen, C. A. (2015). Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood. International Biodeterioration & Biodegradation, 104, 231-237.

Bhattacharjya, D. K., Paul, R. K., Miah, M. N., & Ahmed, K. U. (2015). Comparative study on nutritional composition of oyster mushroom (Pleurotus ostreatus Fr.) cultivated on different sawdust substrates. Bioresearch Communications-(BRC), 1(2), 93-98.

Bonatti, M., Karnopp, P., Soares, H. M., & Furlan, S. A. (2004). Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. Food chemistry, 88(3), 425-428.

Chang, S. T., Lau, O. W., & Cho, K. Y. (1981). The cultivation and nutritional value of Pleurotus sajor-caju. European journal of applied microbiology and biotechnology, 12, 58-62.

Dovbeshko, G., Chegel, V., Gridina, N. Y., Repnytska, O., Shirshov, Y., Tryndiak, V., Todor, I., and Solyanik, G. (2002). Surface enhanced IR absorption of nucleic acids from tumor

cells: FTIR reflectance study.Biopolymers:Original Research on Biomolecules, 67(6), 470-486.

Dubey, D., Dhakal, B., Dhami, K., Sapkota, P., Rana, M., Poudel, NS., and Aryal, L. (2019). Comparative study on effect of different.

Dundar, A., & Yildiz, A. (2009). A comparative study on Pleurotus ostreatus (jacq.) P. kumm. cultivated on different agricultural lignocellulosic wastes. Turkish Journal of Biology, 33(2), 171-179.

Fanadzo, M., Zireva, D. T., Dube, E., & Mashingaidze, A. B. (2010). Evaluation of various substrates and supplements for biological efficiency of Pleurotus sajor-caju and Pleurotus ostreatus. African Journal of Biotechnology, 9(19), 2756-2761.

Frimpong-Manso, J., Obodai, M., Dzomeku, M., & Apetorgbor, M. M. (2011). Influence of rice husk on biological efficiency and nutrient content of Pleurotus ostreatus (Jacq. ex. Fr.) Kummer.

Fufa, B. K., Tadesse, B. A., & Tulu, M. M. (2021). Cultivation of Pleurotus ostreatus on agricultural wastes and their combination. International Journal of Agronomy, 2021, 1-6.

Girmay, Z., Gorems, W., Birhanu, G., & Zewdie, S. (2016). Growth and yield performance of Pleurotus ostreatus (Jacq. Fr.) Kumm (oyster mushroom) on different substrates. Amb Express, 6, 1-7.

Kovath, P. R., Jithenthran, A., Sreelakshmi, N. K., Sriya, V. B., Swathilakshmi, P. S., & Vinni, N. G. (2021). Comparative study on cultivation of oyster mushroom Pleurotus Ostreatus on different substrates. Int. J. Sci. Res. Engin Develop, 4(3), 1639-1643.

Liu, R., Yu, H., & Huang, Y. (2005). Structure and morphology of cellulose in wheat straw. Cellulose, 12, 25-34.

Lun, L. W., Gunny, A. A. N., Kasim, F. H., & Arbain, D. (2017). Fourier transform infrared spectroscopy (FTIR) analysis of paddy straw pulp treated using deep eutectic solvent. In AIP conference proceedings, 1835(1).

Mondal, S. R., Rehana, M. J., Noman, M. S., & Adhikary, S. K. (2010). Comparative study on growth and yield performance of oyster mushroom (Pleurotus florida) on different substrates. Journal of the Bangladesh Agricultural University, 8(452-2016- 35698).

Muswati, C., Simango, K., Tapfumaneyi, L., Mutetwa, M., & Ngezimana, W. (2021). The effects of different substrate combinations on growth and yield of oyster mushroom (Pleurotus ostreatus). International Journal of Agronomy, 2021, 1-10.

Nasreen, Z., Ali, S., Usman, S., Nazir, S., & Yasmeen, A. (2016). Comparative study on the growth and yield of Pleurotus ostreatus mushroom on lignocellulosic by- products. Int. J. Adv. Res. Bot, 2, 42-49.

Naveena, K. C., & Ramalingappa, B.(2019). Isolation Screening, FTIR analysis and GC- MS studies of oleaginous fungi Pleurotus ostreatus. IJRSB. 7(12), 22-26.

Nelson, M.L., O’Connor. (1964). R.T. Relation of certain infrared bands to cellulose crystallinity and crystal lattice type. Part II. A new infrared ratio for estimation of crystallinity in celluloses I and II. J. Appl. Polym. Sci. 8, 1325–1341.

Obodai, M., Cleland-Okine, J., & Vowotor, K. A. (2003). Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by- products. Journal of Industrial Microbiology and Biotechnology, 30(3), 146-149.

Oseni, T. O., Dlamini, S. O., Earnshaw, D. M., & T MASARIRAMBI, M. I. C. H. A. E. L. (2012). Effect of substrate pre-treatment methods on oyster mushroom (Pleurotus ostreatus) production. International journal of agriculture and biology, 14(2).

Oseni, T.O., Dube, S.S., Wahome, P.K., Masarirambi, M.T., &Earnshaw, D.M. (2012). Effect of wheat bran supplement on growth and yield of oyster mushroom (Pleurotus Ostreatus) on fermented pine sawdust substrate. Exp Agric Hortic, 30–40.

Pandey, K. K., & Pitman, A. J. (2003). FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi. International biodeterioration & biodegradation, 52(3), 151-160.

Pokhrel, C.P. (2016). Cultivation of oyster mushroom: a sustainable approach of rural development in Nepal. Journal of Institute of Science and Technology, 21(1): 56- 60.

Ponmurugan, P., Sekhar, Y.,& Sreeshakti, T. (2007). Effect of various substrates on the growth and quality of mushrooms. Pak J Bio Sci,10,171-173.

Pujol, D., Liu, C., Gominho, J., Olivella, M. À., Fiol, N., Villaescusa, I., & Pereira, H. (2013). The chemical composition of exhausted coffee waste. Industrial Crops and Products, 50, 423-429.

Raman, J., Jang, K. Y., Oh, Y. L., Oh, M., Im, J. H., Lakshmanan, H., & Sabaratnam, V. (2021). Cultivation and nutritional value of prominent Pleurotus spp.: an overview. Mycobiology, 49(1), 1-14.

Raspolli Galletti, A. M., D’Alessio, A., Licursi, D., Antonetti, C., Valentini, G., Galia, A., & Nassi o Di Nasso, N. (2015). Midinfrared FT-IR as a tool for monitoring herbaceous biomass composition and its conversion to furfural. Journal of Spectroscopy, 2015.

Sanchez, C. (2010). Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied microbiology and biotechnology, 85, 1321-1337.

Schulz, H., and Baranska, M. (2007). Identification and quantification of valuable plant

substances by IR and Raman spectroscopy. Vibrational Spectroscopy, 43(1),13-25.

Shah, Z. A., Ashraf, M., & Ishtiaq, M. (2004). Comparative study on cultivation and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates (wheat straw, leaves, saw dust). Pakistan Journal of Nutrition, 3(3), 158-160.

Sharma, S., Yadav, R. K. P., & Pokhrel, C. P. (2013). Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. Journal on New Biological Reports, 2(1), 03-08.

Sofi, B., Ahmad, M., & Khan, M. (2014). Effect of different grains and alternate substrates on oyster mushroom (Pleurotus ostreatus) production. African Journal of Microbiology Research, 8(14), 1474-1479.

Swantomo, D., Rochmadi, R., Basuki, K. T., & Sudiyo, R. (2014). Synthesis of smart biodegradable hydrogels cellulose-acrylamide using radiation as controlled release fertilizers. Advanced Materials Research, 896, 296-299.

Thuc, L. V., Corales, R. G., Sajor, J. T., Truc, N. T. T., Hien, P. H., Ramos, R. E., ... & Van Hung, N. (2020). Rice-straw mushroom production. Sustainable rice straw management, 93-109.

Wang, D., Sakoda, A., & Suzuki, M. (2001). Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresource technology, 78(3), 293-300.

Yan, F., Tian, S., Du, K., & Wang, X. (2021). Effects of steam explosion pretreatment on the extraction of xylooligosaccharide from rice husk. BioResources,16(4),691